Coding

Part:BBa_K2185002:Design

Designed by: BRHS iGEM Team   Group: iGEM16_BroadRun-Baltimore   (2016-09-04)


Penicillium alpha amylase coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1436
    Illegal XhoI site found at 1459
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
    Illegal AgeI site found at 250
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 564
    Illegal BsaI site found at 636


Design Notes

Kpn and Spe restriction sites were removed and sequence was optimized for S.cerevisiae.


Source

It comes from the fungus Penicilium. The sequence comes from GenBank.

References